Table 1.
Overall purification of the thermophilic DNase a.
| Fraction | Vol (mL) | Protein content (mg) | Total activity (U) | Sp act (U/mg) | Recovery (%) | Increase in sp act |
|---|---|---|---|---|---|---|
| Crude | 250.0 | 320.0 | 1408.5 | 4.40 | 100 | 1 |
| Concentrated | 39.0 | 35.0 | 1379.26 | 39.41 | 97.9 | 8.96 |
| Sephadex G-50 | 46.2 | 2.8 | 854.98 | 305.35 | 60.70 | 69.40 |
| Membrane | 45 | 0.57 | 365.12 | 640.56 | 25.22 | 145.58 |
a
One unit of enzyme activity is defined as an increase in absorbance of 0.05 units in a cuvette of 1 cm path length at 260 nm using the acid soluble assay at 55 °C.