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. 2014 Jan 21;15(1):1554–1573. doi: 10.3390/ijms15011554

Figure 4.

Figure 4.

Double immunogold labeling for γH2AX (10 nm gold) and BrdU (20 nm gold) in SVZ. (A) Top, double-labeled type C cell with a characteristic deeply indented nucleus and chromatin lax. The areas indicated by the rectangles are shown at higher magnification in the inserts. Bottom, type A cell singularly labeled for BrdU; (B) A BrdU singularly-labeled type C cell (top right: right insert at top) and a double labeled type B cell (center: left insert at top); (C): Double labeled mitotic cell. The area indicated by the rectangle is displayed in the bottom left insert; and (D) Double labeled type C cell. The area in the rectangle is shown at higher magnification in the top right insert. Arrowheads indicate 10 nm gold, arrows 20 nm gold. Abbreviations: γH2AX = phosphorylated form of histone H2AX; BrdU = 5-bromo-2-deoxyuridine; chr = chromosomes; epe = ependyma; he = heterochromatin; LV = lateral ventricle; P = postnatal day. Scale bars: 2 μm; inserts: 100 nm, except top insert in (A) 50 nm.