Table 1.
Semi-quantitative distribution of γH2AX-IR nuclei in the mouse brain. Staining was performed on paraffin sections using the ABC method. The level of immunoreactivity (IR) through the brain was assessed using the following scale: − = no signal; + = weak staining; ++ = moderate staining; +++ = strong staining. Abbreviations: γH2AX = phosphorylated form of histone H2AX; E = embryonic day; OB = olfactory bulb; P = postnatal day; RMS = rostral migratory stream; and SVZ = subventricular zone.
| Distribution of γH2AX-IR nuclei in mouse brain | ||||||||
|---|---|---|---|---|---|---|---|---|
| Age | E 14.5 | P0 | P5 | P10 | P15 | P20 | P60 | Old |
| Forebrain | ||||||||
| Cerebral cortex | − | − | − | − | + | ++ | +++ | +++ |
| SVZ/RMS/OB | +++ | + | + | +++ | ++ | ++ | ++ | ++ |
| Amygdala | − | − | − | − | + | + | ++ | − |
| Hypothalamus | − | − | − | − | − | + | ++ | − |
| Dorsal endopiriform nucleus | − | − | − | + | − | + | + | − |
| Hippocampus | − | − | − | + | + | + | + | − |
| Midbrain | − | − | − | − | + | + | + | − |
| Hindbrain | ||||||||
| Cerebellum | − | + | +++ | +++ | + | − | − | − |
| Pons | − | − | − | − | + | + | + | + |