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Distribution of MNPs 24 h after IVT injection in the left eye of Xenopus embryos. (a–e) Prussian Blue staining on paraffin section. The arrow points to blue labeled MNPs. CNS, central nervous system; E, eye; N, notochord; P, pharynx; H, heart; S, somites; Pr, pronephros; G, gut; (f) Iron content assay on explanted eyes. CL, contralateral eye (non-injected); INJ, injected eyes; K, wild type eyes. Scale bar, 50 μm. n = 3, ** p < 0.001, t-test.
