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. 2014 Jan 22;15(1):1590–1605. doi: 10.3390/ijms15011590

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — Prussian Blue staining on paraffin section of Xenopus embryos at different time points from MNPs injection. MNPs are blue labeled. n = 45 each time point. (a) Three days after injection; (b) five days after injection; (c) 10 days after injection; (d) 20 days after injection. Arrowheads point to MNP localization in interdigitated RPE microvilli with the outer segment of photoreceptors; arrow points to MNPs derived from RPE rupture, during manipulation (details in the material and methods section). Scale bar, 50 μm.