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(a) Reference image with empty rectum, the landmarks are place along the contours of prostate (white) and rectum (yellow); (b) test image with full rectum; (c) result of automatic B-spline registration of the test image onto the reference image, mismatched contours of landmarks are shown in red and green; (d) result of registration by B-spline algorithm and subsequent application of Gaussian RBF with σ = 10 mm and λ = 0.5 for landmark matching.
