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. Author manuscript; available in PMC: 2014 Jan 31.
Published in final edited form as: Bioconjug Chem. 2002 Mar-Apr;13(2):216–223. doi: 10.1021/bc0100657

Figure 6.

Figure 6

Specific targeting of peritoneal macrophages by fMLF. Each BALB/C mouse was injected intraperitoneally with 5 μg of PEG/A–fMLF4–DIG4 (experiment 1, n = 3) or PEG/A–DIG8 (experiment 2, n = 4). After 1 h, the peritoneal cavity was flushed, and cells were collected and lysed as described in Experimental Procedures. ELISA was performed to determine intracellular digoxigenin; a standard curve for digoxigenin was included on every assay plate. The results are normalized to the amount of protein in each lysate, to correct for variations in cell number and efficiency of lysis.