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. Author manuscript; available in PMC: 2014 Jan 31.
Published in final edited form as: J Immunol. 2010 Oct 22;185(11):6572–6583. doi: 10.4049/jimmunol.0904203

FIGURE 5.

FIGURE 5

EBF does not directly inhibit hoxa transcription. A, An EBF−/− cell line stably expressing a tamoxifen-inducible EBF–ER fusion protein were treated with 1 µM 4-HT for 24 h to induced nuclear localization of EBF. After 24 h, the cells were harvested, then analyzed by real-time RT-PCR for transcripts representing established EBF target genes (b29, mb1, pax5), transcripts corresponding to cell surface markers differentially expressed on EBF−/− cells and downregulated as a function of B cell differentiation (flt3, cd34, cd27), or hoxa and meis1 transcripts. The labels indicate Ctr (media control), diluent (95% ethanol), and 4-HT. Data are plotted as the mean ± SD of normalized expression ratios obtained from three independent experiments. B, Downregulation of hematopoietic progenitor markers on EBF−/− cells treated with 4-HT. Three days posttreatment with 4-HT (hatched lines), the EBF−/− cells were harvested and analyzed by flow cytometry for the indicated cell-surface markers. The black line represents EBF−/− cells without 4-HT and the gray filled histogram unstained cells.