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. 2012 Nov 26;8(3):162–170. doi: 10.46582/jsrm.0803009

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Copyright © 2012 Journal of Stem Cells and Regenerative Medicine

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4: Neuronal and astroglial differentiation of induced neural precursor cells. Co-expression of (A) MASH1 and NGN2 and (B) SOX2 and PAX6 in differentiating iNP colonies. (C) iNP-derived neurons express the neuronal markers TUJ1, NSE, and MAP2. Arrows indicate cells exhibiting a typical neuronal morphology. (D) iNP-derived neurons express GAD_65/67 or (E) tyrosine hydroxylase (TH) with NSE co-expression. (F) Image of iNP-derived neuron undergoing patch clamp recording after 30 days of differentiation. (G) Representative trace of action potential generated in response to current injections from iNP-derived neuron after 30 days of differentiation. (H) Astrocytes derived from iNP cells express the astroglial marker GFAP. Scale bars: 25 µm.