Figure 1.

Figure 1: Scheme for hESC cell culture manipulation and methods. (A) Embryoid bodies (EB) were generated from small clumps (3-5 cells) in honeycomb microwells and then transferred to suspension culture. At indicated time points, EBs were dissociated into small clumps (3-5 cells) and plated down as monolayer on gelatin in 96-well flat bottom plate. Overall optimized differentiation schemes for the first 14 days of cardiac induction of H7. The optimized stage-specific differentiation scheme for generating cardiac cells from (B) H7 and (C) H9 hESC.