Figure 1.

The number of nucleotide errors contained in the final transcriptome assembly, normalized to assembly size, is related to the strength of quality trimming. This pattern is largely unchanged with varying depth of sequencing coverage (10–100 million sequencing reads). Trimming at Phred = 5 may be optimal, given the potential untoward effects of more stringent quality trimming. 10, 20, 50, 75, 100 M refer to the subsamples size. 10 M replicate is the technical replicate, 10 M alt. dataset is the secondary dataset. Note that to enhance clarity, the Y-axis does not start at zero.