Figure 3.

The number of unique Blast matches contained in the final transcriptome assembly is related to the strength of quality trimming, with more aggressive trimming resulting in worse performance. Data are normalized to the number of BLAST hits obtained in the most favorable trimming level for each dataset. Negative numbers indicate the detrimental affect of trimming. 10, 20, 50, 75, 100 M refer to the subsamples size. 10 M replicate is the technical replicate, 10 M alt. dataset is the secondary dataset.