Table 1.

The Phred trimming levels that resulted in optimal assemblies across the 4 metrics tested in the different size datasets.

Dataset size	Error	Map	ORF	BLAST
10 M	20	0	2	2
10 M rep.	20	2	2	2
10 M alt	20	2	0	0
20 M	5	5	2	2
50 M	5	10	5	2
75 M	20	10	5	0
100 M	20	0	2	2

Error, the number of nucleotide errors in the assembly. Map, the number of concordantly mapped reads. ORF, the number of ORFs identified. BLAST, the number of unique BLAST hits. 10 M rep. is the technical replicate, 10 M alt. is the secondary dataset.