Fig. 3.

Tollip knockdown increases HLA-DR expression. (A) HeLa-CIITA-DO cells were transfected with control or TOLLIP-specific siRNAs, and cultured for 48 h at 37 °C. The bar chart represents the mRNA expression of Tollip for cells transfected with specific or control siRNA. (B) The cells were stained and analyzed by flow cytometry for cell surface and total expression of HLA-DR (L243 Ab). The mean fluorescence values (MFV) were plotted to account for variations in the levels of HLA-DR. Error bars represent standard deviation obtained for two different transfections. (C) Cells were stained for cell surface expression of CLIP (CerCLIP) and total expression of invariant chain (BU45). The mean fluorescence values (MFV) were plotted to account for variations in the levels of CLIP and invariant chain Error bars represent standard deviation obtained for two different transfections. (D) HeLa-DR1 and HeLa-DR1 TM/TM cells were transfected with control or Tollip-specific siRNAs, and cultured for 48 h in 37 °C. Cells were stained for cell surface expression of HLA-DR (L243 Ab). The mean fluorescence values (MFV) were plotted to account for variations in the levels of HLA-DR expression. Error bars represent standard deviation obtained for two different transfections. Data is representative of at least two different experiments.