Skip to main content
. 2013 Feb 20;3:17–25. doi: 10.1016/j.rinim.2013.02.002

Fig. 5.

Fig. 5

Tollip reduces the expression of MARCH1. (A) HeLa CIITA and HEK 293E CIITA cells were transfected with EYFP-MARCH1, GFP-Tollip or an empty vector (mock). Cell lysates were blotted for actin (asterisk) and MARCH1. The intensity of the bands was quantified, normalized to actin and divided by the one of cells transfected with MARCH1. Results are represented as a bar chart. (B) HeLa CIITA cells were transfected with EYFP-MARCH1, GFP-Tollip or an empty vector (mock). Cell lysates were blotted for Tollip. The intensity of the bands was quantified and the value obtained for cells expressing Tollip alone was set to 1. Results are represented as a bar chart. (C) HeLa CIITA or HEK 293E CIITA cells were transfected with EYFP-MARCH1 (left panel) of EYFP-MARCH1K-0 (right panel) with or without GFP-Tollip, GFP-SOCS1 and EYFP. Cells were stained for cell surface MHC II and analysed by flow cytometry. The mean fluorescence values for MHC II in cells expressing Tollip and EYFP was set to 1. Data is representative of a least two different experiments.