Table 1.

Bacterial strains and plasmids

Strains or plasmids	Genotype or description	Source or reference
E. coli strains
E. coli TOP10	F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG; used for general cloning	Invitrogen
M. tb. strains
H37Rv	M. tuberculosis WT strain	Oatway & Steenken (1936)
ΔpknF	H37Rv with deletion of pknF constructed by homologous recombination with targeting construct pRW51	Spivey et al. (2011)
pknF complement	ΔpknF containing complementing plasmid pRW95	Spivey et al. (2011)
ΔRv1747	H37Rv with deletion of Rv1747 constructed by homologous recombination with targeting construct pRW69	Curry et al. (2005)
Rv1747 complement	ΔRv1747 containing complementing plasmid pRW76	Curry et al. (2005)
M. tuberculosis shuttle plasmids
p2Nil	Suicide gene delivery vector, oriE, KanR	Hinds et al. (1999)
pKP186	Integrase negative derivative of the integrating vector pMV306, KanR	Papavinasasundaram et al. (2001)
pBS-Int	Suicide vector containing integrase, AmpR	Springer et al. (2001)
pEJ414	pMV306 derivative containing a promoterless E. coli lacZ reporter gene, KanR	Papavinasasundaram et al. (2001)
pRW69	p2Nil containing a 2-kb region of H37Rv DNA flanking each side of the Rv1747 gene, HygR	Curry et al. (2005)
pRW76	Rv1747 complementing plasmid. pKP186 derivative containing 609 bp Rv1745c,pknF and Rv1747, KanR HygR	Curry et al. (2005)
pRW51	p2Nil containing a 1.5-kb region of H37Rv DNA flanking each side of the pknF gene	Spivey et al. (2011)
pRW95	pknF complementing plasmid. pKP186 derivative containing 609 bp Rv1745c,pknF and 20 bp of Rv1747, KanR	Spivey et al. (2011)
pVS_01	pEJ414 containing pknF promoter region, KanR	This work