Figure 1.

Transfer plating from different locations of the colony result in different patterns.

A. P. vortex’s colony, grown on peptone agar (20 g l⁻¹ peptone, 2.25% w/v agar) for 96 h at 30°C. The colours were inverted to emphasize higher cell densities using the brighter shades of yellow.

v, vortices; b, branches; i, inoculation zone.

B. Demonstrating replica plating from the vortices, grown on peptone for 48 h at 30°C. The colony is characterized by accelerated expansion rate (∼ 0.098 mm h⁻¹) in comparison to mixed colony expansion rate (∼ 0.0625 mm h⁻¹) (Fig. S1).

C. Replica plating from inoculation zone, grown on peptone for 48 h at 30°C. The colony has shown no expansion as opposed to isolations from vortices.