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. 2013 Jun 14;15(9):2532–2544. doi: 10.1111/1462-2920.12160

Figure 4.

Figure 4

Morphotypes stability.A. Reversion of builders into the mixed culture. Builders’ enriched culture, obtained after × 4 transfers at the early log phase, was grown in liquid LB for 8 h, 12 h and 24 h. At each time point, samples were taken and builders’ percentage in the culture was evaluated. Builders are stable for at least 8 h (I,II). At 12 h, they revert back to the mixed culture (III), remaining ∼ 50% of the culture.B. Shows the stability of explorers’ morphotype after different treatments. Explorers’ microcolonies were inoculated in liquid LB O.N. (I) Samples were re-inoculated in liquid LB for 48 h; (II) Samples were re-inoculated in liquid LB for 24 h, five times continuously; (III) samples were inoculated as a colony on LB agar. After each treatment, explorers’ percentage in the culture was evaluated. The first two treatments (I and II) resulted in over 90% explorers in the culture. Only plating on hard medium induced the reversion to the mixed culture composition.