TABLE 1.
Inter-spin distance in BAK in solution state and in the membrane-inserted state
Average distances between the indicated spin label pairs (first column) introduced in BAK mutants shown in Fig. 2 are summarized for the solution (third column) and membrane-inserted (fourth column) states in this table. The Cβ-Cβ distances for the original amino acid pairs are shown in the second column.
| Spin label pairs | Cβ-Cβ distance in solution structure | Inter-spin distance in solution (±S.D) by DEER or CW | Inter-spin distance in membrane (±S.D.) by DEER or CW |
|---|---|---|---|
| Å | Å | Å | |
| 30/75 (α1/α2) | 16.9 | 26.6 (± 2.9) | >50 |
| 30/154 (α1/α6) | 8.1 | 9.0 (± 6.5) (by CW) | >50 |
| 55/146 (α1–2 loop/α5–6 loop) | 9.9 | 13.7 (± 5.3) (by CW) | 49.6 (± 23.8) |
| 75/122 (α2/ α5 N-terminal) | 16.4 | 21.5 (± 3.4) | 27.1 (± 6.3) |
| 83/122 (α2–3 loop/α5 N-terminal) | 11.2 | 12.2 (± 4.4) (by CW) | 21.5 (± 2.6) |
| 83/135 (α2–3 loop/α5) | 15.3 | 26.4 (± 7.0) | 10.6 (± 5.4) (by CW) |
| 99/146 (α3–4 loop/α5–6 loop) | 15.2 | 23.3 (± 3.3) | 23.6 (± 5.5) |
| 107/154 (α4/α6) | 15.2 | 22.6 (± 6.6) | 30.5 (± 6.7) |
| 107/164 (α4/α6) | 21.3 | 20.9 (± 3.5) | 40.1 (± 12.8) |
| 122/164 (α5 N-terminal/α6 N-terminal) | 13.2 | 22.6 (± 5.1) | > 50 |
| 135/154 (α5/α6) | 16.7 | 25.2 (± 5.8) | 30.7 (± 12.5) |
| 135/162 (α5/α6) | 21.8 | 27.7 (± 2.2) | 36.9 (± 10.5) |
| 135/164 (α5/α6) | 23.6 | 33.0 (± 5.0) | 38.9 (± 10.5) |