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. 2013 Dec 18;289(5):2600–2609. doi: 10.1074/jbc.M113.534636

FIGURE 5.

FIGURE 5.

Rich2-mediated functional morphogenesis of dendritic spines. Hippocampal neurons were transfected at DIV-9 with the indicated plasmids, in combination with YFP, and tested at DIV-12. A, portions of dendrites showing spines (arrowheads) of hippocampal neurons transfected with GFP alone (control), or in combination with either Rich2, si-Rich2 or si-Rich2 + Rich2-mt (scale bar: 1 μm). The right images show enlarged views of the square areas. B and C, histograms show changes in the size of spine head and spine density in GFP-transfected neurons (control), and neurons transfected with either Rich2, si-control or si-Rich2 or si-Rich2 + Rich2-mt (*, p < 0.001). D and G, sample traces of whole-cell patch-clamp recordings obtained from cultured hippocampal neurons transfected with the indicated plasmids at DIV-9, and tested at DIV-12. E and F, frequency and amplitude histograms of mEPSCs obtained from neurons transfected with GFP alone (control) or in combination with Rich2 (n = 10 in each condition, *, p < 0.05). H and I, same legend as for E, F, but from neurons transfected with si-Rich2.