FIGURE 2.
Degradation of cellulose and cello-oligosaccharides by NcLPMO9C. A shows overlaid HPAEC chromatograms of oligosaccharides released upon degradation of 1 mg/ml of PASC, Avicel, and steam-exploded spruce with 8.8 μm NcLPMO9C. B shows products released upon incubation of 0.4 mg/ml cello-oligosaccharides with 4.4 μm NcLPMO9C (Glcn traces), a standard mixture of nonoxidized cello-oligosaccharides (DP2–5, Glc2–5 trace), as well as products released upon incubation of cellopentaose with 4.4 μm NcLPMO9C and 1.4 μm MtCDH (CDH trace). All reaction mixtures contained 2 mm ascorbic acid (except the one containing CDH) and 5 mm ammonium acetate buffer, pH 6.0, and were incubated for 24 h at 50 °C. Reactions with insoluble substrates were incubated with horizontal shaking at 850 rpm. For product nomenclature, see Fig. 1. See text for further details.