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. 2013 Dec 11;289(5):2687–2700. doi: 10.1074/jbc.M113.502641

FIGURE 7.

FIGURE 7.

The increased IL-8, CCL2, and CXCL5 gene expression induced by proteasome inhibition in ovarian cancer cells is mediated by EGR-1. A, Western analysis of EGR-1 and control actin levels in whole cell extracts prepared from OVCAR3 cells transfected with control and EGR-1-specific siRNA and treated 24 h with 0, 0.1, and 10 μm BZ. B, densitometric evaluation of EGR-1 levels in whole cell extracts of OVCAR3 cells transfected with control and EGR-1 siRNA as described in A. The EGR-1 band densities were normalized to actin. The data represent the mean of three experiments ± S.E. C, real time RT-PCR analysis of IL-8, CCL2, CXCL5, IL-6, and TNFα mRNA levels in OVCAR3 cells transfected with control or EGR-1 siRNA and incubated 24 h with 0, 0.1 and 10 μm BZ. The values represent the mean ± S.E. of four experiments; asterisks denote a statistically significant (p < 0.05) inhibition compared with cells transfected with control siRNA. UT, untreated.