FIGURE 5.

Hhp2 kinase activity is required for Sre1N degradation. A, Western blot of whole-cell lysates from sre1N hhp2Δ cells carrying empty vector, hhp2⁺, or hhp2-K41N constructs and sre1N cells with empty vector. Blots were probed for Sre1N, Myc, and β-actin expression and are representative of three independent experiments. B, Western blot of whole-cell lysates from sre1N or sre1N hhp2Δ cells carrying empty vector or hhp2⁺ plasmid treated with DMSO or 1 mm bortezomib (BZ) for 3 h in the presence of oxygen. Lanes 3–6 show two independent plasmid transformants. Blots were probed for Sre1N expression and are representative of three independent experiments.