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Concentration-dependent PHD induction by rosiglitazone. C3H10T1/2 cells were treated with the indicated concentrations of rosiglitazone for 10 days, and the extent of adipocyte differentiation was determined by Oil Red O staining (A), RT-PCR (B), and Western blotting (C). Experiments were conducted three times, and representative results are shown. Densitometric analysis was conducted using UN-SCAN-IT Gel version 5.1 software (Silk Scientific). C, control; R, rosiglitazone.
