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. 2013 Dec 12;289(5):2765–2775. doi: 10.1074/jbc.M113.537043

FIGURE 1.

FIGURE 1.

Transport activity of MCT1 is enhanced by CAIV in a non-catalytic manner. A, original recordings of [H+]i in Xenopus oocytes either expressing MCT1+CAIV (upper trace) or MCT1 alone (lower trace), during application of 3 and 10 mm lactate in the nominal absence and in the presence of 5% CO2/10 mm HCO3− (pHo 7.0) before and during application of 10 μm EZA. Rate of change in [H+]i induced by application (B, C) and removal (D, E) of lactate in oocytes expressing MCT1 and MCT1+CAIV, respectively, in the nominal absence (B, D) or presence (C, E) of 5% CO2/10 mm HCO3− in the absence and presence of 10 μm EZA. F, rate of change in [H+]i induced by application of lactate in oocytes expressing MCT1, either alone or together with CAIV-WT or the catalytically inactive mutant CAIV-V165Y, in the nominal absence of CO2/HCO3−. G, rate of change in [H+]i induced by application of 5% CO2/10 mm HCO3− in oocytes expressing MCT1, MCT1+CAIV-WT or MCT1+CAIV-V165Y. Asterisks at the bars for MCT1+CAIV-coexpressing oocytes refer to the values of MCT1-expressing cells.