FIGURE 4.

Fluorescence staining of MCT1, MCT4, and CAIV in oocytes coexpressing MCT1+CAIV (A) and MCT4+CAIV (D), respectively (upper rows). For control MCT1- and MCT4-expressing oocytes were stained against CAIV and CAIV-expressing oocytes were stained against MCT1 or MCT4. Native oocytes were double-stained against MCT1/4 and CAIV (lower rows). B, Western blot for CAIV, MCT1 and β-tubulin (as loading control) in native (N), MCT1- (M), and MCT1+CAIV-expressing oocytes (M+C). C, quantification of the protein level of MCT1 in MCT1+CAIV-expressing oocytes relative to the protein level in oocytes expressing MCT1 alone. E, Western blot for CAIV, MCT4 and β-tubulin (as loading control) in native (N), MCT4- (M), and MCT4+CAIV-expressing oocytes (M+C). F, quantification of the protein level of MCT4 in MCT4+CAIV-expressing oocytes relative to the protein level in oocytes expressing MCT4 alone.