TABLE 1.

Primers used in this study

F, forward; R, reverse; q, quantitative.

Primers used to clone PfN44 to the expression vector
PfN44-e-5	GATCCATGGGCTTTTGTTACAAAACATTTGG
PfN44-e-3	CATCTCGAGAAATCCCGGGAAATCAGCAAATTTC
Primers used for RACE
RACE3	TTCCCTCTACTATGAAGACAAG
RACE5	GCCGTTGATTGGCTGTCC
LONG	CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT
SHORT	CTAATACGACTCACTATAGGGC
NUP	AAGCAGTGGTATCAACGCAGAGT
Primers used for full-length clone
FULL1	AATCTCATACGGAGAAATTGGTGT
FULL2	GAATGACACACATTTTTTATTGTA
Primers used for RNAi
RNAi-P1	CTAATACGACTCACTATAGGGAGAAGGAATTCTATTTTTGATGC
RNAi-P2	CTAATACGACTCACTATAGGGAGAAGGGTTTATTGTAATGTTCG
Primers used for RT-PCR
RT-PfN44-F	GAGGTCGGAGAAGTAAG
RT-PfN44-R	GAACACGATGGTAAAAA
RT-GAPDH-F	GCCGAGTATGTGGTAGAATC
RT-GAPDH-R	CACTGTTTTCTGGGTAGCTG
Primers for real time
qβ-actin-f	CTCCTCACTGAAGCCCCCCTC
qβ-actin-r	ATGGCTGGAATAGGGATTCTGG
qKRMP-F	AAGAAATGTCACCCTTGGGATTGG
qKRMP-R	AATCATCGCCACCATATCCATCG
qNacrein-F	GGCTTTGGCGACGAACCGGA
qNacrein-R	ACACGGGGGAGTGGTCAGGG
qPfN44-F	TTGCTGATGGATGTGAAG
qPfN44-R	CTGTATATTGCCGTTGATTG