Repressing cardiac PI3K abolished the cardio-protective effects of ARIA deletion.
A, phosphorylation of Akt in the hearts of WT, ARIA-KO, dnPI3K-Tg, and ARIA-KO/dnPI3K-Tg mice (n = 8). *, p < 0.01 versus WT mice. **, p < 0.01 versus ARIA-KO mice. #, not significant versus dnPI3K-Tg mice. B, cardiac fibrosis was evaluated by Masson's trichrome staining (n = 5–6). *, p < 0.01 versus sham-treated dnPI3K-Tg mice. **, p < 0.01 versus DOX-treated dnPI3K-Tg mice. ***, p < 0.01 versus DOX-treated ARIA-KO mice. #, not significant versus DOX-treated dnPI3K-Tg mice. Bar = 100 μm. C, cardiomyocyte apoptosis was evaluated by TUNEL staining (n = 5). *, p < 0.01 versus ARIA-KO mice. **, p < 0.05 versus ARIA-KO mice. ***, p < 0.05 versus dnPI3K-Tg mice.