FIGURE 1.

Characterization of autophagy hallmarks in GCN5L1 MEFs. A, schematic representation of alleles produced by GCN5L1 gene-targeting construct. B, genotype and protein expression in wild type, hemizygous, and homozygous knock-out GCN5L1 MEF cells. C, electron micrograph of GCN5L1 wild type and knock-out MEF cells. Autophagosomes are marked by an asterisk. D and E, transcript and active lipidated protein form (II) of LC3 are increased in GCN5L1 knock-out MEFs. This can be reversed by the reconstitution of GCN5L1 expression.