FIGURE 2.

Expression of autophagy pathway mediators in GCN5L1 MEFs. A, GCN5L1 knock-out MEFs show no protein expression defects in either the ATG or mTOR autophagy pathways. B and C, expression of the autophagy-related transcription factor TFEB, along with its targets p62 and LAMP1, is increased in GCN5L1 knock-out MEFs. D, degradation and accumulation of LAMP1, p62, and LC3 in cells treated with cycloheximide (CHX) and Bafilomycin A1 (Baf A1) at the noted concentrations for 4 or 20 h. E, absolute and relative turnover of p62 following treatment with Bafilomycin A1 and cycloheximide for 6 h. F, proteins involved in autophagy are present in the correct cellular compartment in both wild type and knock-out GCN5L1 MEFs. WCL, whole cell lysate; Cyto, cytoplasm; HM, heavy membrane. G and H, reconstitution of GCN5L1 in knock-out MEF cells reverses the increased expression of TFEB and its targets.