TABLE 1.

Peptides used in fluorescence polarization assay

Hsp70/90 C-terminal peptides used in fluorescence polarization assays are shown here. For binding experiments, each peptide tracer/probe contained a 5-carboxyfluorescein-labeled amino terminus. Labels were connected to peptides through an aminohexanoic acid linker. Additionally, for competitive binding experiments, each unlabeled peptide competitor contained a free amino-terminal end. Mutant residues in the C-terminal tracer are in boldface and underlined type.

C-terminal peptide sequences	Corresponding human chaperones
MEEVD	Hsp90α/β
IEEVD	Hsc70 and Hsp72
DDTSRMEEVD	Hsp90α
EDASRMEEVD	Hsp90β
SSGPTIEEVD	Hsc70
GSGPTIEEVD	Hsp72
DVEEM	Reverse peptide control
DDTSRIEEVD	Hsp90α mutant
GSGPTMEEVD	Hsp72 mutant