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The effects of various culture conditions on the intracellular localization of Gat1 in wild type and double Gat1 substitution mutants. The plasmids used in this experiment were wild type (pKA62) and double Gat1 substitution mutants M40L,M95A (pRR1063), M40L,M102A (pRR1061), and M95A,M102A (pRR1079). The experimental format and data presentation were as in Fig. 4.
