FIGURE 1.
Complementation study on CL production of E. coli BKT12 strain expressing cls genes from B. pseudofirmus 811M. B. pseudofirmus 811M cls genes were cloned into pBAD-TOPO vector and overexpressed in CL-deficient E. coli BKT12 with 0.2% arabinose induction overnight in the presence of [32P]PO4. Lipids were extracted and analyzed as described under “Experimental Procedures.” The test plasmids are pBAD-Bp-ClsA (A), pBAD-Bp-ClsB (B), pBAD-Bp-ClsC (C), and pBAD-TOPO/lacZ/V5-His (D) as a control. The values shown are representative of three determinations.