TABLE 2.
Mass spectrometry characterization of the NTCB cleavage products
The five fragments shown in Fig. 3A were cut from the gel and digested with trypsin, and the resulting peptides were identified by mass spectrometry. The molecular masses and peptide fingerprints of the five NTCB fragments are consistent with cleavage of the Cys462-Cys541 disulfide bond in a fraction of plasminogen.
| Band | Predicted residues | Representative peptide |
|---|---|---|
| 2 | Glu1-Asp461 | Residues 118–134; FSPATHPSEGLEENYCR |
| 3 | Cys462-Asn791 | Residues 494–504; HSIFTPETNPR |
| 4 | Cys541-Asn791 | Residues 662–677; VIPACLPSPNYVVADR |
| 5 | Cys462-Gln540 | Residues 494–504; HSIFTPETNPR |