TABLE 1.
Microarray and Q-PCR analysis of mRNA abundance after 24-h chase
Q-PCR analysis used the ΔΔCt method relative to the average of “FA only” replicates (=1.0). The Q-PCR was not performed if the last three columns are empty.
| Microarray analysisa |
Q-PCR analysis (FA+LPS)/FA |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Unstimulated | FA only | LPS only | FA+LPS | Ratio FA/unstimulated | Ratio (FA+LPS)/LPS | Ratio (FA+LPS)/FA | Mean | S.D. | nb | |
| Fatty acid uptake | ||||||||||
| Acsl1 | 112 | 101 | 621 | 854 | 0.90 | 1.4 | 8.5 | 19.5 | 6.3 | |
| Acsl4 | 156 | 136 | 113 | 141 | 0.87 | 1.2 | 1.0 | 1.3 | 1 | |
| Acsl5 | 66 | 80 | 83 | 69 | 1.20 | 0.8 | 0.9 | 2.2 | 1 | |
| CD36 | 135 | 192 | 63 | 89 | 1.42 | 1.4 | 0.5 | 0.2 | 2 | |
| Slc27a1 (FATP1) | 244 | 259 | 254 | 280 | 1.07 | 1.1 | 1.1 | 0.5 | 0.2 | 4 |
| Slc27a4 (FATP4) | 87 | 91 | 95 | 105 | 1.05 | 1.1 | 1.2 | |||
| Slc27a6 (FATP6) | 91 | 83 | 54 | 54 | 0.91 | 1.0 | 0.7 | |||
| Fabp3 | 243 | 214 | 897 | 773 | 0.88 | 0.9 | 3.6 | 9.5 | 4.2 | 4 |
| Fabp4 (aP2) | 62 | 60 | 50 | 52 | 0.87 | 1.0 | 0.9 | |||
| Fabp5 (mal1) | 505 | 519 | 114 | 151 | 0.87 | 1.3 | 0.3 | |||
| TAG synthesis | ||||||||||
| Dgat1 | 73 | 86 | 72 | 83 | 1.18 | 1.2 | 1.0 | 0.6 | 1 | |
| Dgat2 | 50 | 48 | 60 | 66 | 0.98 | 1.1 | 1.4 | 8.6 | 4.2 | 5 |
| Lpgat1 | 59 | 55 | 53 | 52 | 0.93 | 1.0 | 0.9 | |||
| Glucose uptake | ||||||||||
| Slc2a1 (GLUT1) | 832 | 816 | 1833 | 1847 | 0.87 | 1.0 | 2.3 | |||
| Acylglycerolphosphate synthesis | ||||||||||
| Gyk | 129 | 124 | 98 | 99 | 0.96 | 1.0 | 0.8 | 0.9 | 1 | |
| PEPCK | 61 | 58 | 58 | 57 | 0.94 | 1.0 | 1.0 | |||
| Agpat2 | 283 | 247 | 281 | 256 | 0.87 | 0.9 | 1.0 | |||
| Agpat6 | 990 | 950 | 1272 | 1232 | 0.96 | 1.0 | 1.3 | |||
| Agpat9 (GPAT3) | 60 | 59 | 62 | 67 | 0.99 | 1.1 | 1.1 | |||
| Lipid droplet proteins | ||||||||||
| Plin2 | 851 | 1194 | 267 | 854 | 1.40 | 3.2 | 0.7 | 0.7 | 0.3 | 7 |
| Triglyceride lipolysis | ||||||||||
| Pnpla2 (ATGL) | 1852 | 2257 | 1155 | 1242 | 1.22 | 1.1 | 0.6 | 0.5 | 0.1 | 3 |
| Lipe | 60 | 69 | 67 | 64 | 1.15 | 1.0 | 0.9 | 1.2 | 2 | |
| Mgll | 400 | 432 | 83 | 88 | 1.08 | 1.1 | 0.2 | 0.1 | 0.04 | 3 |
| G0S2 | 63 | 60 | 61 | 59 | 0.95 | 1.0 | 1.0 | |||
| Abhd5 | 333 | 323 | 180 | 232 | 0.97 | 1.3 | 0.7 | 1.0 | 1 | |
| Mitochondrial fatty acid uptake, function | ||||||||||
| Cpt1a | 56 | 58 | 54 | 54 | 1.04 | 1.0 | 0.9 | 0.8 | 0.3 | 3 |
| Ppargc1b | 99 | 113 | 82 | 111 | 1.14 | 1.4 | 1.0 | 1.2 | 1 | |
| Regulatory proteins | ||||||||||
| Hif1a | 105 | 102 | 114 | 140 | 0.97 | 1.2 | 1.4 | 1.85 | 0.21 | 5 |
| Sirt1 | 149 | 148 | 119 | 118 | 1.00 | 1.0 | 0.8 | 0.6 | 2 | |
| Pparg | 812 | 731 | 87 | 106 | 0.90 | 1.2 | 0.1 | 0.1 | 0.1 | 3 |
| Ampk (Prkaa1) | 60 | 60 | 64 | 60 | 1.00 | 0.9 | 1 | |||
| Nfe2l2 | 404 | 339 | 181 | 169 | 0.84 | 0.9 | 0.5 | 0.5 | 1 | |
a Means are of four independent experiments. LPS = 10 ng/ml. Cells were studied 48 h after starting FA load and 24 h after adding LPS.
b n = number of independent experiments, each with 3 replicates/condition.