TABLE 1.

Structural statistics for the solution structure of TePixJ GAF domain as Pb

Restraints and structural statistics	r.m.s.d.
	〈Sa〉a	Lowest energy
Experimental 1H-1H distance restraints
GAF domain (Å) (2847)
Intraresidue (1017)	0.091 ± 0.003	0.090
Sequential |i − j| = 1 (772)	0.063 ± 0.004	0.058
Short range 1 < |i − j| ≤ 5 (575)	0.123 ± 0.015	0.125
Long range |i − j| > 5 (483)	0.242 ± 0.026	0.239
PVB (Å) (163)
Intraresidue (67)	0.295 ± 0.003	0.295
Sequential |i − j| = 1 (26)	0.031 ± 0.022	0.040
Short range 1 < |i − j| ≤ 5 (38)	0.420 ± 0.019	0.392
Long range |i − j| > 5 (32)	0.436 ± 0.091	0.603
Hydrogen bonds (Å) (136)	0.095 ± 0.007	0.087
Predicted dihedral restraints (°) (294)
ϕ (147) / ψ (147)	2.02 ± 0.50	1.95
Residual dipolar couplings (Hz) (217)
1DNH Pf1 (128) Rb = 18.2%	1.43 ± 0.03	1.44
1DCαHα Pf1 (89) Rc = 31.2%	2.50 ± 0.04	2.56
Deviations from idealized covalent geometry
Bonds (Å) (2738)	0.0014 ± 0.0001	0.0014
Angles (°) (4946)	0.398 ± 0.001	0.402
Impropers (°) (1498)	0.85 ± 0.02	0.86
Coordinate precisiond
Backbone (N, Cα, C')	0.20 ± 0.04	0.16
All non-hydrogen atoms	0.62 ± 0.04	0.58

^a 〈S_a〉 represents the 10 lowest-energy structures. For 〈S_a〉, each value represents the mean± S.D. with the number of restraints used to calculate these values shown in parentheses.

^b R factor is defined as in XPLOR-NIH (28).

^c R and r.m.s.d. of C^αH^α RDCs are scaled to NH.

^d Average r.m.s.d. of the 10 lowest energy structures from the mean coordinates for structured residues 12–50, 72–83 and 94–148.