TABLE 2.

Structural statistics for the solution structure of TePixJ GAF domain as Pg

Restraints and structural statistics	r.m.s.d.
	〈Sa〉a	Lowest energy
Experimental 1H-1H distance restraints
GAF domain (Å) (2444)
Intraresidue (879)	0.182 ± 0.004	0.182
Sequential |i − j| = 1 (599)	0.083 ± 0.004	0.079
Short range 1 < |i − j| ≤ 5 (468)	0.116 ± 0.010	0.119
Long range |i − j| > 5 (498)	0.113 ± 0.008	0.111
PVB (Å) (62)
Intraresidue (22)	0.486 ± 0.030	0.471
Sequential |i − j| = 1 (3)	0.0 ± 0.0	0.0
Short range 1 < |i − j| ≤ 5 (9)	0.130 ± 0.022	0.134
Long range |i − j| > 5 (28)	0.152 ± 0.048	0.115
Hydrogen bonds (Å) (140)	0.082 ± 0.005	0.083
Predicted dihedral restraints (°) (272)
ϕ (136)/ψ (136)	2.16 ± 0.08	2.19
Residual dipolar couplings (Hz) (201)
1DNH Pf1 (109) Rb = 10.2%	1.30 ± 0.04	1.44
1DCαHα Pf1 (92) Rc = 23.2%	3.04 ± 0.06	3.00
Deviations from idealized covalent geometry
Bonds (Å) (2742)	0.0014 ± 0.0001	0.0014
Angles (°) (4954)	0.373 ± 0.002	0.370
Impropers (°) (1496)	0.79 ± 0.01	0.78
Coordinate precisiond
Backbone (N, Cα, C')	0.33 ± 0.12	0.29
All non-hydrogen atoms	0.73 ± 0.10	0.63

^a 〈S_a〉 represents the 10 lowest-energy structures. For 〈S_a〉, each value represents the mean ± S.D. with the number of restraints used to calculate these values shown in parentheses.

^b R factor is defined as in XPLOR-NIH (28).

^c R and r.m.s.d. of C^αH^α RDCs are scaled to NH.

^d Average r.m.s.d. of the 10 lowest energy structures from the mean coordinates for structured residues 12–50, 72–83, and 94–148.