FIGURE 3.

PK activity is not altered by the NaCl concentration. The effect of NaCl on the intrinsic activity of PK was assessed using purified hamster recPrP as substrate. Soluble, folded recPrP was dialyzed for 4 h at room temperature and supplemented with different concentrations of NaCl ranging from 0 to 1000 mm. Samples were treated with increasing PK concentrations at 37 °C for 30 min at 600 rpm agitation. PK-digested samples were immunoblotted as specified under “Experimental Procedures.”