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Effect of different salts on PrPSc protease resistance. Pure PrPSc purified in the absence of salt was supplemented with sodium bromide (NaBr), sodium chloride (NaCl), calcium chloride (CaCl2), or potassium chloride (KCl) at 150 mm and sodium fluoride (NaF) at 96 mm (saturation at the reaction temperature). Reactions were submitted to protease digestion (1000 μg/ml PK) followed by immunoblotting.
