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In vitro replication of PrPSc by PMCA. PrPSc purified with or without 150 mm NaCl was serially diluted into normal brain homogenate and used as inoculum for seeding PMCA as described under “Experimental Procedures.” Tubes were incubated for 42 h (84 PMCA cycles), and samples were treated with PK except for the normal brain homogenate (NBH) used as a migration control.
