FIGURE 7.
Effect of salts on protease resistance of PrPSc obtained from different prion strains and species. Two different prion strains from hamster (263K and Hyper) and three from mouse (ME7, RML, and 301C) were assayed for protease resistance in the presence (+) or absence (−) of 150 mm NaCl. Samples were PK-digested for 4 h at 37 °C at protease concentrations optimal for detectability of each prion strain: 600 μg/ml (263K, Hyper, and 301C) and 200 μg/ml (ME7 and RML). Dotted lines separate samples that are coming from different blots.