Fig. (2).

In vitro assay. (A) Effect of AEGH on lymphocyte cell viability. Lymphocytes were incubated with AEGH at indicated concentrations for 48 h. Each bar represents mean ± SEM. Treatment with AEGH did not affect cell viability. (B) Effect of AEGH on lymphocyte proliferation induced by ConA [5 μg/ml] and treated with or without AEGH [80 μg/ml] for 48 h. Each bar represents mean ± SEM (n=6). Cells incubated only with RPMI medium were used as unstimulated controls. *P < 0.05 versus ConA stimulated but non-treated cells, by Student’s unpaired t-test. (C). Effect of AEGH on lymphocyte proliferation induced by LPS. Lymphocytes were incubated with LPS [10 μg/ml] and treated with or without AEGH [80 μg/ml] for 48 h. Each bars represents mean ± SEM (n=6). Cells incubated only with RPMI medium was used as unstimulated control. *P < 0.05 versus LPS stimulated but nontreated cells, by Student’s unpaired t-test.