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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Breast Cancer Res Treat. 2013 Dec 6;143(1):113–124. doi: 10.1007/s10549-013-2789-2

Figure 4. Recruitment of serine-5 and serine-2 -phosphorylated RNA polymerase II at the cMYC promoter.

Figure 4

(A) Schematic presentation of cMYC promoter showing the transcription start site (TSS). The grey box represents the region (~150bp upstream of TSS) probed using real-time PCR following ChIP assay. (B) Recruitment of serine-2 phosphorylated RNA polymerase II and (C) serine-5 phosphorylated RNA polymerase II was assessed by ChIP assay followed by real-time PCR in MCF7: and MCF7:5C cells. Values are represented as percent input of the starting chromatin, adjusted for control IgM recruitment for each sample. (* p<.05 versus MCF7 cells) (D) Total protein levels of serine-2 and serine-5 phosphorylated RNA polymerase II in MCF7 and MCF7:5C cells.