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. 2013 Sep 27;11(5):312–319. doi: 10.1016/j.gpb.2013.09.003

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© 2013 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Production and hosting by Elsevier B.V. All rights reserved.

This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Neuronal and glial differentiation of rat iNS cells A. Phase contrast image of neurons spontaneously differentiated from rat iNS cells after the removal of LIF/CHIR/Y. B. Tuj1 and GFAP immunostaining of cells generated from rat iNS cells after exposure to EGF and FGF2 for 10 days followed by culturing in N2B27 medium plus 1% serum for another 7 days. C. Exposure to PDGF-AA and T3 (triiodothyronine) induced differentiation of rat iNS cells toward Rip-positive oligodendrocytes. D–H. Different subtypes of neurons derived from rat iNS cells. Scale bar, 50 μm.