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. 2013 Sep 27;11(5):312–319. doi: 10.1016/j.gpb.2013.09.003

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© 2013 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Production and hosting by Elsevier B.V. All rights reserved.

This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure S2 — Characterization of membrane properties and neurotransmitter phenotype of neurons derived from rat iNS cells (A) Injection of current steps into iPS-derived neurons elicits action potentials (APs). (B) Spontaneous AP firing of an iPS-derived neuron. Inset, expanded view of a single AP. (C) Some cells show both spontaneous excitatory postsynaptic currents (EPSCs) and spontaneous inhibitory postsynaptic currents (IPSCs). (D) Isolated miniature EPSC (top panel) in the presence of tetrodotoxin (TTX) and bicuculline (Bicu) can be further blocked by AMPA receptor antagonist CNQX (lower panel), indicating that the neuron is glutamatergic in nature.