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. Author manuscript; available in PMC: 2014 Jan 31.
Published in final edited form as: Immunity. 2012 Dec 27;38(1):79–91. doi: 10.1016/j.immuni.2012.12.001

Figure 2. Dual origins of macrophages.

Figure 2

(A) (i) Flow cytometric and histological analysis of livers obtained from Cx3cr1cre/+:R26-rfp:Cx3cr1gfp/+ mice. In these mice GFP expression acts as a direct reporter for CX3CR1 expression, while RFP expression is controlled by Cre recombinase. (ii) Analysis of fetal liver and adult liver and Kupffer cells of Cx3cr1gfp/+ mice.

(B) Flow cytometric analysis of the intestinal lamina propria of DTx-treated Cd11c-dtr mice that received 7 days earlier a Ly6C+ YFP monocyte graft isolated from tamoxifen-treated Cx3cr1creER/+:R26-yfp mice. Results are representative of two experiments involving 3 mice per group.

(C) Flow cytometric analysis of peritoneal lavages of Cx3cr1creER/+:R26-rfp mice administered a single tamoxifen gavage (5 mg) one day following an intra-peritoneal thioglycollate injection. Rightmost graph shows phenotypic shift of monocyte-derived cells between three weeks and 8 weeks. Results are representative of two independent experiments involving 3 mice per group.