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. Author manuscript; available in PMC: 2014 Jan 31.
Published in final edited form as: Int J Cancer. 2008 Jun 1;122(11):2462–2470. doi: 10.1002/ijc.23383

Figure 3.

Figure 3

Oncogenic K-ras induces differential effects on proliferation and apoptosis in the distal and proximal colon. Distal and proximal colonic epithelium from LSL-K-rasG12D mice (LSL) and LSL-K-rasG12D/Vil-Cre/+ mice (LSL/cre) were evaluated for BrdU incorporation and TUNEL-staining cells by immunohistochemistry. (a) Proliferative cells were detected by incorporation of BrdU. The proliferative index (number of BrdU-labeled cells/colonic crypt) is plotted for distal and proximal colon. (b) Cells undergoing apoptosis were detected by TUNEL staining. The apoptotic index (number of apoptotic cells/100 colonic crypts) is plotted for distal and proximal colon. Values represent mean ± SEM; n = 4–5 animals/group. (c) Distal and proximal colonic epithelium was isolated from LSL and LSL/cre mice and assayed for active (GTP-bound) Ras using the Ras-binding domain of Raf-1 as an affinity ligand. Active and total K-ras were detected by immunoblot analysis with an antibody specific for K-ras. Blot is representative of 3 independent assays.