Skip to main content
. Author manuscript; available in PMC: 2014 Jan 31.
Published in final edited form as: Nat Commun. 2013;4:2429. doi: 10.1038/ncomms3429

Figure 3. Hyperpolarized 2-HG production is detected in mutant IDH1 cell lysates.

Figure 3

(a) 13C MR spectra acquired 45s after injection of hyperpolarized [1-13C] α-KG in U87IDHmut cell lysate (top) and U87IDHwt cell lysate (bottom) (absolute intensities; * contaminant originating from the α-KG preparation). (b) gHMBC spectra of the same U87IDHmut lysates acquired at 14 Tesla, confirming the production of [1-13C] 2-HG from [1-13C] α-KG. The 1H-13C cross peaks of [1-13C] 2-HG (dotted box), confirm the [1-13C] 2-HG assignment at 183.9ppm. Note: [1-13C] glycine was used as an external reference. (c) Temporal evolution of [1-13C] 2-HG in U87IDHmut cell lysate normalized to [5-13C] α-KG peak intensity, showing build-up of hyperpolarized [1-13C] 2-HG.