FIG. 1.
Increased p62 and LC3-II protein expression in FXR KO mouse livers. A, Total lysates from 3- and 6-month-old FXR+/+ and FXR−/− mouse livers were subjected to immunoblot analysis. Digital data are densitometry analysis and presented as a ratio of wild type mice (n = 4–5). B, mRNA was isolated from around 3-month-old FXR+/+ and FXR−/− KO mouse livers and qRT-PCR was performed. The gene expression levels were normalized to β-actin and shown as fold increase over wild type mice (n = 6–7). C, Total liver lysates from 3-month-old FXRfl/fl:Alb-Cre- and FXRfl/fl:Alb Cre+ mouse livers were subjected to immunoblot and densitometry analysis.