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BAs did not directly affect lysosomal or proteasome functions. A, Primary hepatocytes were pre-loaded with DQ-BSA (5 µg/ml) for 1hr and then treated with BAs for 6h. Fluorescence intensity from cleaved DQ-BSA was measured using total cell lysates. Hepatocytes were treated with BAs for 6h, and total cell lysates were either incubated with z-RR-AMC for cathepsin B activity (B) or Suc-LLVY-AMC with or without MG132 (2 µm) for proteasome activity measurement (C) (n = 3).
